In vitro study on skin-related bioactivity of Pleurotus cystidiosus extract

Abstract

Pleurotus cystidiosus, distinguished for its nutritional and biomedical attributes due to its rich repository of bioactive compounds, presents promising therapeutic functionalities for cosmetic applications. This study investigated the anti-photoaging and moisturizing potential of P.cystidiosus extract (PCE) for cosmetic application, addressing a gap in the scientific knowledge regarding the use of P.cystidiosus in cosmetic formulations. Through an extraction process by 60% saturation with ammonium sulfate, PCE1, PCE2, and PCE3 were obtained, exhibiting the method's efficiency with high protein yields and a rich presence of short molecular weight peptides, as confirmed by the Lowry assay and electrophoresis. The extract demonstrated antioxidant activity, assessed via 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,20 -azino-bis-3- ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging assays, alongside anti-tyrosinase activity, primarily attributed to PCH (≤3kDa). Non-anti-bacterial activity of S. aureus, S. epidermidis, and C. acnes. was observed. Particularly, PCE exhibited UV radiation-protective effects against photoaging in Human keratinocytes (HaCaT) cells at 100 µg/mL concentration, suggesting its potential for sunscreen and anti-aging product integration. This study also investigated the skin moisturizing effect of PCE in cosmetic formulations through snake skin moisturizing studies and water contact angle measurements, revealing an improvement in skin wettability. These results highlighted the critical role of PCE in formulating moisturizing products, integral to skincare routines.

-