Effects of Roll-on Containing Cannabis Extract and Isolated cannabidiol on nociception in mice

Abstract

Inflammation is a common cause of pain, associated with injuries, infections, diseases, or other health problem which declines in quality of life. Cannabis contains biological active compounds such as delta-9-tetrahydrocannabinol (Δ-9-THC) and cannabidiol (CBD). CBD has many beneficial pharmacological effects, including antinociception and anti‐inflammation. This study aimed to investigate the antinociceptive and anti-inflammatory effects of the roll-on containing cannabis extract and isolated CBD on muscle pain models induced by carrageenan in male mice. Mice were applied the roll-on isolated CBD or cannabis extracts (0.5% and 1%) on gastrocnemius muscle for 19 hr. Then 3% carrageenan was used to induce muscle pain before evaluating nociceptive behavior elicited by heat and mechanical insults by using the hotplate test and von Frey filament test, respectively. Inflammatory markers (TNFα and IL-1β) and structural changes in muscle were measured by specific ELISA and Crystal violet staining, respectively. The results showed that mice treated with 1% roll-on isolated CBD, and 1% roll-on cannabis extract decreased mechanical (von Frey test) and thermal hyperalgesia (hot plate test) as well as reduced inflammatory markers TNF-α, IL-1β, and inflammatory cells. These results demonstrated that 1% roll-on cannabis extract and CBD reduced pain by modulating the inflammatory cytokines consequently alleviate the inflammatory cells.

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