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Title: Isolation and expression analysis of B- and E-class MADS Box genes in regulation of perianth formation in Rhynchostylis retusa (L.) Blume
การแยกและวิเคราะห์การแสดงออกของยีน MADS BOX กลุ่ม B และ E ในการควบคุมการพัฒนาของกลีบดอกกล้วยไม้เอื้องไอยเรศ
สุดารัตน์ เกษมชลธาร
Pattamon Sangin
พัทธมน แสงอินทร์
Naresuan University. Faculty of Science
Keywords: DEF-like genes
AGL6-like genes
Floral development
MADS- box genes
Rhynchostylis retusa
Issue Date: 2020
Publisher: Naresuan University
Abstract: Rhynchostylis retusa (L.) Blume is a popular and scarce orchid species with attractive flowers arranged in a racemose cluster. To study this flower’s complex floral perianth formation, MADS-box genes that play an important role in flower morphogenesis were investigated. Four B-class DEF-like MADS-box genes (RrDEF1, RrDEF2, RrDEF3, and RrDEF4) and two E-class AGL6-like MADS-box genes (RrAGL6-1 and RrAGL6-2) were identified, and their expression was characterized by qRT-PCR. These genes were expressed in every developmental stage of reproductive organs but not in the vegetative leaves, except for RrDEF2. Most of these genes revealed similar expression patterns in stage 3 and mature flowers. Higher RrDEF1 and RrAGL6-1 expression was detected in the sepals and petals rather than the lips, while RrDEF3, RrDEF4, and RrAGL6-2 were highly expressed in the lips but exhibited low expression in the sepals. RrDEF3 was also strongly expressed in petals. These gene expression patterns supported current profiles involving sepal/petal/lip determination, with RrDEF1 and RrAGL6-1 promoting sepal/petal formation, while RrDEF3, RrDEF4, and RrAGL6-2 promoted lip formation. Our findings support revised ‘orchid code’ and ‘perianth code’ hypotheses in which floral organs are regulated by combined levels of expression from each clade of DEF-like genes and AGL6-like genes. These four RrDEF and two RrAGL6 genes promote and enrich our knowledge and understanding concerning the floral development of orchids.
Description: Master of Science (M.S.)
วิทยาศาสตรมหาบัณฑิต (วท.ม.)
Appears in Collections:คณะวิทยาศาสตร์

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