ผลของการเคลือบผิวโพลี(เมทิลเมทาคริเลต)ด้วยอนุภาคนาโนเงินด้วยวิธีที่ต่างกันต่อลักษณะพื้นผิวและการเกาะติดของแบคทีเรีย

Abstract

Poly(methylmethacrylate) (PMMA) is an acrylic resin widely used as a denture base material for the Thai elderly for a long time. However, there are still some structural limitations that promote microbial adhesion. Silver nanoparticles (AgNPs) were recently introduced as a surface coating because of their antimicrobial effect. Development of the surface coating with the AgNPs is an attractive strategy to improve the denture's hygiene.

This study aims to compare the effects of PMMA surface coating methods with the AgNPs on PMMA’s surface roughness, characteristics of AgNPs, and bacterial adhesion.

Materials and methods: Three different AgNP coating methods (simple immersion, ultrasound irradiation, and microwave irradiation) were used. Six PMMA discs of each method were prepared and compared surface roughness values (Ra) between pre and post-coating and three methods (n=6). The characteristics of AgNPs were also evaluated by using FE-SEM. Fifty PMMA discs were divided into five groups (Negative control, Positive control, Simple immersion, Ultrasound irradiation, and Microwave irradiation) (n=10). The specimens were incubated in S. aureus suspension and incubated at 37 ºc for 3 hours. Then bacterial adhesion analysis was evaluated by the spread plate method.

Results: There were differences in the surface roughness between pre and post-coating and among the three methods. The simple immersion method provided AgNPs with small size and regularly distributed nanospheres. In comparison, the AgNPs from ultrasound and microwave methods were varying size nanospheres and agglomerations. There were differences in the bacterial adhesion between the selected AgNPs coating methods compared to the control. The simple immersion method allowed a better reduction of bacterial adhesion than other methods. Nevertheless, there were no differences in the bacterial adhesion between ultrasound and microwave methods.

Conclusion: Different AgNP coating methods affect different surface roughness of PMMA and the shape, size, and distribution patterns of the AgNPs. Interestingly, the simple immersion method provided the most significant bacterial reduction.

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